Human PIP4K2B/PIP4KII-beta Recombinant Protein (N-His) (HX081012)

Name: Human PIP4K2B/PIP4KII-beta Recombinant Protein (N-His)
Brand: abinScience
SKU: HX081012
Price: 211 USD
Availability: InStock

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Numéro de catalogue	HX081012
Description	Recombinant Human PIP4K2B/PIP4KII-beta Protein, N-His (HX081012) expressed in E. coli, spanning Ser2-Thr416. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P78356
Longueur de protéine	Ser2-Thr416
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	48.49 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	1-phosphatidylinositol 5-phosphate 4-kinase 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, PtdIns(5)P-4-kinase isoform 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, PIP4KII-beta, Diphosphoinositide kinase 2-beta, PI(5)P 4-kinase type II beta, PIP5K2B, PIP4K2B
Contexte	Phosphatidylinositol 5-phosphate 4-kinase type-2 beta (PIP4K2B/PIP4KII-beta) is a ~47 kDa protein. Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Preferentially utilizes GTP, rather than ATP, for PI(5)P phosphorylation and its activity reflects changes in direct proportion to the physiological GTP concentration. Its GTP-sensing activity is critical for metabolic adaptation. PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism. They interact with PIP5Ks and suppress PIP5K-mediated PtdIns(4,5)P2 synthesis and insulin-dependent conversion to PtdIns(3,4,5)P3. 1. Sumita, K. et al. (2016) Molecular cell 61, 187-98. PMID: 26774281 2. Castellino, AM. et al. (1997) The Journal of biological chemistry 272, 5861-70. PMID: 9038203 3. Wang, DG. et al. (2019) Cell reports 27, 1991-2001.e5. PMID: 31091439
Note	For research use only.

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SDS-PAGE for Recombinant Human PIP4K2B/PIP4KII-beta Protein, N-His

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Human PIP4K2B/PIP4KII-beta Recombinant Protein (N-His).pdf

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