Human PIP4K2B/PIP4KII-beta Recombinant Protein (N-His) (HX081011)

Name: Human PIP4K2B/PIP4KII-beta Recombinant Protein (N-His)
Brand: abinScience
SKU: HX081011
Price: 298 USD
Availability: InStock

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Overview

Catalog No.	HX081011
Description	Recombinant Human PIP4K2B/PIP4KII-beta Protein, N-His (HX081011) expressed in Mammalian Cells, spanning Ser2-Thr416. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian cells
Accession	P78356
Protein length	Ser2-Thr416
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	48.49 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	1-phosphatidylinositol 5-phosphate 4-kinase 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type-2 beta, PtdIns(5)P-4-kinase isoform 2-beta, Phosphatidylinositol 5-phosphate 4-kinase type II beta, PIP4KII-beta, Diphosphoinositide kinase 2-beta, PI(5)P 4-kinase type II beta, PIP5K2B, PIP4K2B
Background	Phosphatidylinositol 5-phosphate 4-kinase type-2 beta (PIP4K2B/PIP4KII-beta) is a ~47 kDa protein. Participates in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. Preferentially utilizes GTP, rather than ATP, for PI(5)P phosphorylation and its activity reflects changes in direct proportion to the physiological GTP concentration. Its GTP-sensing activity is critical for metabolic adaptation. PIP4Ks negatively regulate insulin signaling through a catalytic-independent mechanism. They interact with PIP5Ks and suppress PIP5K-mediated PtdIns(4,5)P2 synthesis and insulin-dependent conversion to PtdIns(3,4,5)P3. 1. Sumita, K. et al. (2016) Molecular cell 61, 187-98. PMID: 26774281 2. Castellino, AM. et al. (1997) The Journal of biological chemistry 272, 5861-70. PMID: 9038203 3. Wang, DG. et al. (2019) Cell reports 27, 1991-2001.e5. PMID: 31091439
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human PIP4K2B/PIP4KII-beta Protein, N-His

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Datasheet

Human PIP4K2B/PIP4KII-beta Recombinant Protein (N-His).pdf

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Formula

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Human PIP4K2B/PIP4KII-beta Recombinant Protein (N-His) (HX081011)

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Datasheet

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