Human PLA2G1B Recombinant Protein (N-His) (HY504012)

Name: Human PLA2G1B Recombinant Protein (N-His)
Brand: abinScience
SKU: HY504012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HY504012
Description	Recombinant Human PLA2G1B Protein, N-His (HY504012) expressed in E. coli, spanning Asp16-Ser148. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P04054
Longueur de protéine	Asp16-Ser148
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	17.15 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Phospholipase A2, PLA2G1B, Group IB phospholipase A2, PLA2A, PPLA2, Phosphatidylcholine 2-acylhydrolase 1B, PLA2
Contexte	Phospholipase A2 (PLA2G1B) is a ~16 kDa protein. Secretory calcium-dependent phospholipase A2 that primarily targets dietary phospholipids in the intestinal tract. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. May play a role in the biosynthesis of N-acyl ethanolamines that regulate energy metabolism and inflammation in the intestinal tract. Hydrolyzes N-acyl phosphatidylethanolamines to N-acyl lysophosphatidylethanolamines, which are further cleaved by a lysophospholipase D to release N-acyl ethanolamines. May act in an autocrine and paracrine manner. 1. Suzuki, N. et al. (2000) The Journal of biological chemistry 275, 5785-93. PMID: 10681567 2. Kanda, A. et al. (1992) Biochimica et biophysica acta 1171, 1-10. PMID: 1420353 3. Yu, BZ. et al. (2007) Biochimica et biophysica acta 1768, 2478-90. PMID: 17603006 4. Pan, Y. et al. (2014) Scientific reports 4, 6660. PMID: 25335547 5. Ancian, P. et al. (1995) The Journal of biological chemistry 270, 8963-70. PMID: 7721806
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human PLA2G1B Protein, N-His

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Human PLA2G1B Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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