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| カタログ番号 | HB944012 |
|---|---|
| 説明 |
Recombinant Human PLA2G2A Protein, N-His (HB944012) expressed in E. coli, spanning Asn21-Cys144. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P14555 |
| タンパク質長 | Asn21-Cys144 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 16.21 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Phosphatidylcholine 2-acylhydrolase 2A, NPS-PLA2, RASF-A, Non-pancreatic secretory phospholipase A2, Group IIA phospholipase A2, GIIC sPLA2, PLA2L, PLA2G2A, PLA2B, Phospholipase A2, membrane associated |
| 背景 | Phospholipase A2, membrane associated (PLA2G2A) is a ~16 kDa protein. Secretory calcium-dependent phospholipase A2 that primarily targets extracellular phospholipids with implications in host antimicrobial defense, inflammatory response and tissue regeneration. Hydrolyzes the ester bond of the fatty acyl group attached at sn-2 position of phospholipids (phospholipase A2 activity) with preference for phosphatidylethanolamines and phosphatidylglycerols over phosphatidylcholines. Contributes to lipid remodeling of cellular membranes and generation of lipid mediators involved in pathogen clearance. Displays bactericidal activity against Gram-positive bacteria by directly hydrolyzing phospholipids of the bacterial membrane. Upon sterile inflammation, targets membrane phospholipids of extracellular mitochondria released from activated platelets, generating free unsaturated fatty acids such as arachidonate that is used by neighboring leukocytes to synthesize inflammatory eicosanoids such as leukotrienes. 1. Ishizaki, J. et al. (1999) The Journal of biological chemistry 274, 24973-9. PMID: 10455175 2. Suzuki, N. et al. (2000) The Journal of biological chemistry 275, 5785-93. PMID: 10681567 3. Kramer, RM. et al. (1989) The Journal of biological chemistry 264, 5768-75. PMID: 2925633 4. Laine, VJ. et al. (1999) Journal of immunology (Baltimore, Md. : 1950) 162, 7402-8. PMID: 10358193 5. Koduri, RS. et al. (2002) The Journal of biological chemistry 277, 5849-57. PMID: 11694541 6. Boudreau, LH. et al. (2014) Blood 124, 2173-83. PMID: 25082876 7. Sun, YX. et al. (2004) The Biochemical journal 380, 749-56. PMID: 14998370 8. Saegusa, J. et al. (2008) The Journal of biological chemistry 283, 26107-15. PMID: 18635536 9. Fujita, M. et al. (2015) The Journal of biological chemistry 290, 259-71. PMID: 25398877 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human PLA2G2A Protein, N-His
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