Human POLB Recombinant Protein (N-His) (HY116012)

Name: Human POLB Recombinant Protein (N-His)
Brand: abinScience
SKU: HY116012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HY116012
Description	Recombinant Human POLB Protein, N-His (HY116012) expressed in E. coli, spanning Asn12-Glu335. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	P06746
Longueur de protéine	Asn12-Glu335
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	39.20 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	DNA polymerase beta, POLB
Contexte	DNA polymerase beta (POLB) is a ~38 kDa protein. Repair polymerase that plays a key role in base-excision repair. During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase); through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase. 1. Iwanaga, A. et al. (1999) Mutation research 435, 121-8. PMID: 10556592 2. Bennett, RA. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 7166-9. PMID: 9207062 3. Matsumoto, Y. et al. (1998) Biochemistry 37, 6456-64. PMID: 9572863 4. Lang, T. et al. (2007) Molecular and cellular biology 27, 5587-96. PMID: 17526740 5. Prasad, R. et al. (1998) The Journal of biological chemistry 273, 11121-6. PMID: 9556598 6. Prasad, R. et al. (1998) The Journal of biological chemistry 273, 15263-70. PMID: 9614142
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human POLB Protein, N-His

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Human POLB Recombinant Protein (N-His).pdf

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Formula

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