Anti-Human POLB Polyclonal Antibody (HY116014)

Name: Anti-Human POLB Polyclonal Antibody
Brand: abinScience
SKU: HY116014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HY116014

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human POLB (Asn12-Glu335).

Target

DNA polymerase beta, POLB

Purification

Purified by antigen affinity column.

Accession

P06746

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA polymerase beta (POLB) is a ~38 kDa protein. Repair polymerase that plays a key role in base-excision repair. During this process, the damaged base is excised by specific DNA glycosylases, the DNA backbone is nicked at the abasic site by an apurinic/apyrimidic (AP) endonuclease, and POLB removes 5'-deoxyribose-phosphate from the preincised AP site acting as a 5'-deoxyribose-phosphate lyase (5'-dRP lyase); through its DNA polymerase activity, it adds one nucleotide to the 3' end of the arising single-nucleotide gap. Conducts 'gap-filling' DNA synthesis in a stepwise distributive fashion rather than in a processive fashion as for other DNA polymerases. It is also able to cleave sugar-phosphate bonds 3' to an intact AP site, acting as an AP lyase.

1. Iwanaga, A. et al. (1999) Mutation research 435, 121-8. PMID: 10556592

2. Bennett, RA. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 7166-9. PMID: 9207062

3. Matsumoto, Y. et al. (1998) Biochemistry 37, 6456-64. PMID: 9572863

4. Lang, T. et al. (2007) Molecular and cellular biology 27, 5587-96. PMID: 17526740

5. Prasad, R. et al. (1998) The Journal of biological chemistry 273, 11121-6. PMID: 9556598

6. Prasad, R. et al. (1998) The Journal of biological chemistry 273, 15263-70. PMID: 9614142

Note

For research use only.

Images

References

Datasheet

Anti-Human POLB Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human POLB Polyclonal Antibody (HY116014)

Overview

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References

Datasheet

SDS

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