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| カタログ番号 | HB094012 |
|---|---|
| 説明 |
Recombinant Human POLD1 Protein, N-GST (HB094012) expressed in E. coli, spanning Met41-Phe254. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P28340 |
| タンパク質長 | Met41-Phe254 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 50.23 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | 3'-5' exodeoxyribonuclease, DNA polymerase subunit delta p125, POLD1, DNA polymerase delta catalytic subunit, POLD |
| 背景 | DNA polymerase delta catalytic subunit (POLD1) is a ~123 kDa protein. As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4. 1. Li, H. et al. (2006) The Journal of biological chemistry 281, 14748-55. PMID: 16510448 2. Meng, X. et al. (2009) Nucleic acids research 37, 647-57. PMID: 19074196 3. Meng, X. et al. (2010) Biochemistry 49, 3545-54. PMID: 20334433 4. Terai, K. et al. (2013) The Journal of biological chemistry 288, 30509-30514. PMID: 24022480 5. Lin, SH. et al. (2013) DNA repair 12, 922-35. PMID: 24035200 6. Conde, CD. et al. (2019) The Journal of clinical investigation 129, 4194-4206. PMID: 31449058 7. Ogi, T. et al. (2010) Molecular cell 37, 714-27. PMID: 20227374 8. Costantino, L. et al. (2014) Science (New York, N.Y.) 343, 88-91. PMID: 24310611 9. Maga, G. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 18850-5. PMID: 24191025 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human POLD1 Protein, N-GST
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