Anti-POLD1 Polyclonal Antibody (HB094014)

Name: Anti-POLD1 Polyclonal Antibody
Brand: abinScience
SKU: HB094014
Price: 168 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HB094014

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human POLD1 (Met41-Phe254).

Target

3'-5' exodeoxyribonuclease, DNA polymerase subunit delta p125, POLD1, DNA polymerase delta catalytic subunit, POLD

Purification

Purified by antigen affinity column.

Accession

P28340

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA polymerase delta catalytic subunit (POLD1) is a ~123 kDa protein. As the catalytic component of the trimeric (Pol-delta3 complex) and tetrameric DNA polymerase delta complexes (Pol-delta4 complex), plays a crucial role in high fidelity genome replication, including in lagging strand synthesis, and repair. Exhibits both DNA polymerase and 3'- to 5'-exonuclease activities. Requires the presence of accessory proteins POLD2, POLD3 and POLD4 for full activity. Depending upon the absence (Pol-delta3) or the presence of POLD4 (Pol-delta4), displays differences in catalytic activity. Most notably, expresses higher proofreading activity in the context of Pol-delta3 compared with that of Pol-delta4.

1. Li, H. et al. (2006) The Journal of biological chemistry 281, 14748-55. PMID: 16510448

2. Meng, X. et al. (2009) Nucleic acids research 37, 647-57. PMID: 19074196

3. Meng, X. et al. (2010) Biochemistry 49, 3545-54. PMID: 20334433

4. Terai, K. et al. (2013) The Journal of biological chemistry 288, 30509-30514. PMID: 24022480

5. Lin, SH. et al. (2013) DNA repair 12, 922-35. PMID: 24035200

6. Conde, CD. et al. (2019) The Journal of clinical investigation 129, 4194-4206. PMID: 31449058

7. Ogi, T. et al. (2010) Molecular cell 37, 714-27. PMID: 20227374

8. Costantino, L. et al. (2014) Science (New York, N.Y.) 343, 88-91. PMID: 24310611

9. Maga, G. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 18850-5. PMID: 24191025

Note

For research use only.

Images

References

Datasheet

Anti-POLD1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-POLD1 Polyclonal Antibody (HB094014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us