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| カタログ番号 | HD558012 |
|---|---|
| 説明 |
Recombinant Human POLD4 Protein, N-GST & C-His (HD558012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9HCU8 |
| タンパク質長 | Glu45-Leu107 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | DNA polymerase delta subunit 4, DNA polymerase delta subunit p12, POLD4, POLDS |
| 背景 | DNA polymerase delta subunit 4 is a ~12 kDa protein. As a component of the tetrameric DNA polymerase delta complex (Pol-delta4), plays a role in high fidelity genome replication and repair. Within this complex, increases the rate of DNA synthesis and decreases fidelity by regulating POLD1 polymerase and proofreading 3' to 5' exonuclease activity. Pol-delta4 participates in Okazaki fragment processing, through both the short flap pathway, as well as a nick translation system. Under conditions of DNA replication stress, required for the repair of broken replication forks through break-induced replication (BIR), a mechanism that may induce segmental genomic duplications of up to 200 kb. Involved in Pol-delta4 translesion synthesis (TLS) of templates carrying O6-methylguanine or abasic sites. 1. Li, H. et al. (2006) The Journal of biological chemistry 281, 14748-55. PMID: 16510448 2. Meng, X. et al. (2009) Nucleic acids research 37, 647-57. PMID: 19074196 3. Meng, X. et al. (2010) Biochemistry 49, 3545-54. PMID: 20334433 4. Lin, SH. et al. (2013) DNA repair 12, 922-35. PMID: 24035200 5. Costantino, L. et al. (2014) Science (New York, N.Y.) 343, 88-91. PMID: 24310611 6. Terai, K. et al. (2013) The Journal of biological chemistry 288, 30509-30514. PMID: 24022480 |
| 注意事項 | For research use only |
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