Human POMT1 Recombinant Protein (N-His) (HV576012)

Name: Human POMT1 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV576012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HV576012
Description	Recombinant Human POMT1 Protein, N-His (HV576012) expressed in E. coli, spanning Pro318-His513. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q9Y6A1
Longueur de protéine	Pro318-His513
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	24.72 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	POMT1, Dolichyl-phosphate-mannose--protein mannosyltransferase 1, Protein O-mannosyl-transferase 1
Contexte	Protein O-mannosyl-transferase 1 (POMT1) is a ~84 kDa protein. Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient. Essentially dedicated to O-mannosylation of alpha-DAG1 and few other proteins but not of cadherins and protocaherins. 1. Beltrán-Valero de Bernabé, D. et al. (2002) American journal of human genetics 71, 1033-43. PMID: 12369018 2. Manya, H. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 500-5. PMID: 14699049 3. Larsen, ISB. et al. (2017) The Journal of biological chemistry 292, 11586-11598. PMID: 28512129
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human POMT1 Protein, N-His

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Human POMT1 Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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Volume

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g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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