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Anti-POMT1 Polyclonal Antibody (HV576014)

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Overview
Catalog No.HV576014
Description
Anti-POMT1 Polyclonal Antibody (HV576014) is a rabbit polyclonal antibody detecting POMT1 in ELISA, IHC, WB. Suitable for Human, Mouse, and Rat.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
  • ●Multi-Species — Cross-reactive for translational research.
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human POMT1 (Pro318-His513).
Target POMT1, Dolichyl-phosphate-mannose--protein mannosyltransferase 1, Protein O-mannosyl-transferase 1
Purification Purified by antigen affinity column.
Accession Q9Y6A1
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Protein O-mannosyl-transferase 1 (POMT1) is a ~84 kDa protein. Transfers mannosyl residues to the hydroxyl group of serine or threonine residues. Coexpression of both POMT1 and POMT2 is necessary for enzyme activity, expression of either POMT1 or POMT2 alone is insufficient. Essentially dedicated to O-mannosylation of alpha-DAG1 and few other proteins but not of cadherins and protocaherins.

1. Beltrán-Valero de Bernabé, D. et al. (2002) American journal of human genetics 71, 1033-43. PMID: 12369018
2. Manya, H. et al. (2004) Proceedings of the National Academy of Sciences of the United States of America 101, 500-5. PMID: 14699049
3. Larsen, ISB. et al. (2017) The Journal of biological chemistry 292, 11586-11598. PMID: 28512129
Note For research use only.
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Datasheet
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-POMT1 Polyclonal Antibody

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