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| カタログ番号 | HW678012 |
|---|---|
| 説明 |
Recombinant Human PPARG Protein, N-His (HW678012) expressed in E. coli, spanning Asp230-Tyr505. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P37231 |
| タンパク質長 | Asp230-Tyr505 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 33.82 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | NR1C3, Nuclear receptor subfamily 1 group C member 3, Peroxisome proliferator-activated receptor gamma, PPAR-gamma, PPARG |
| 背景 | Peroxisome proliferator-activated receptor gamma (PPARG) is a ~57 kDa protein. Ligand-activated transcription factor that forms obligate heterodimers with the retinoic acid receptor and acts as a key regulator of biological processes, such as adipocyte differentiation, lipid metabolism, glucose homeostasis and beta-oxidation of fatty acids. Activated by lipid ligands: binds peroxisome proliferators, such as hypolipidemic drugs, and fatty acids, such as prostaglandin J2 metabolites. Ligand-binding results in a conformational change in the receptor, promoting dissociation of repressors and recruitment of coactivators, and subsequent activation of target gene expression. Specifically binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses. 1. Yin, Y. et al. (2006) Molecular endocrinology (Baltimore, Md.) 20, 268-78. PMID: 16150867 2. Park, SH. et al. (2010) The Journal of biological chemistry 285, 35330-9. PMID: 20829347 3. Katano-Toki, A. et al. (2013) Molecular endocrinology (Baltimore, Md.) 27, 769-80. PMID: 23525231 4. Elbrecht, A. et al. (1996) Biochemical and biophysical research communications 224, 431-7. PMID: 8702406 5. Lambe, KG. et al. (1996) European journal of biochemistry 239, 1-7. PMID: 8706692 6. Mukherjee, R. et al. (1997) The Journal of biological chemistry 272, 8071-6. PMID: 9065481 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human PPARG Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
