Anti-PPARG Polyclonal Antibody (HW678014)

Name: Anti-PPARG Polyclonal Antibody
Brand: abinScience
SKU: HW678014
Price: 96 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HW678014

Description

Anti-PPARG Polyclonal Antibody (HW678014) is a rabbit polyclonal antibody detecting PPARG in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PPARG (Asp230-Tyr505).

Target

NR1C3, Nuclear receptor subfamily 1 group C member 3, Peroxisome proliferator-activated receptor gamma, PPAR-gamma, PPARG

Purification

Purified by antigen affinity column.

Accession

P37231

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Peroxisome proliferator-activated receptor gamma (PPARG) is a ~57 kDa protein. Ligand-activated transcription factor that forms obligate heterodimers with the retinoic acid receptor and acts as a key regulator of biological processes, such as adipocyte differentiation, lipid metabolism, glucose homeostasis and beta-oxidation of fatty acids. Activated by lipid ligands: binds peroxisome proliferators, such as hypolipidemic drugs, and fatty acids, such as prostaglandin J2 metabolites. Ligand-binding results in a conformational change in the receptor, promoting dissociation of repressors and recruitment of coactivators, and subsequent activation of target gene expression. Specifically binds to DNA specific PPAR response elements (PPRE) and modulates the transcription of its target genes, such as acyl-CoA oxidase. Acts as a critical regulator of gut homeostasis by suppressing NF-kappa-B-mediated pro-inflammatory responses.

1. Yin, Y. et al. (2006) Molecular endocrinology (Baltimore, Md.) 20, 268-78. PMID: 16150867

2. Park, SH. et al. (2010) The Journal of biological chemistry 285, 35330-9. PMID: 20829347

3. Katano-Toki, A. et al. (2013) Molecular endocrinology (Baltimore, Md.) 27, 769-80. PMID: 23525231

4. Elbrecht, A. et al. (1996) Biochemical and biophysical research communications 224, 431-7. PMID: 8702406

5. Lambe, KG. et al. (1996) European journal of biochemistry 239, 1-7. PMID: 8706692

6. Mukherjee, R. et al. (1997) The Journal of biological chemistry 272, 8071-6. PMID: 9065481

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-PPARG polyclonal antibody at 1ug/mL on recombinant PPARG (Catalog No. HW678012).

References

Datasheet

Anti-PPARG Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-PPARG Polyclonal Antibody (HW678014)

Overview

Images

References

Datasheet

SDS

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