Human PPARGC1A Recombinant Protein (N-His) (HP446012)

Name: Human PPARGC1A Recombinant Protein (N-His)
Brand: abinScience
SKU: HP446012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HP446012
Description	Recombinant Human PPARGC1A Protein, N-His (HP446012) expressed in E. coli, spanning Ser636-Arg798. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q9UBK2
Longueur de protéine	Ser636-Arg798
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	22.11 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	LEM6, PGC1A, PPARGC1, Ligand effect modulator 6, PGC-1-alpha, PPAR-gamma coactivator 1-alpha, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PPARGC-1-alpha, PPARGC1A, PGC1
Contexte	Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) is a ~91 kDa protein. Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Acts as a key regulator of gluconeogenesis: stimulates hepatic gluconeogenesis by increasing the expression of gluconeogenic enzymes, and acting together with FOXO1 to promote the fasting gluconeogenic program. 1. Knutti, D. et al. (2000) Molecular and cellular biology 20, 2411-22. PMID: 10713165 2. Dominy, JE. et al. (2010) Biochimica et biophysica acta 1804, 1676-83. PMID: 20005308 3. Shin, JH. et al. (2011) Cell 144, 689-702. PMID: 21376232 4. Mays, SG. et al. (2017) Molecular pharmacology 92, 1-11. PMID: 28363985 5. Seacrist, CD. et al. (2020) Structure (London, England : 1993) 28, 830-846.e9. PMID: 32433991 8. Cho, Y. et al. (2013) The Journal of biological chemistry 288, 25207-25218. PMID: 23836911
Note	For research use only.

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SDS-PAGE for Recombinant Human PPARGC1A Protein, N-His

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Human PPARGC1A Recombinant Protein (N-His).pdf

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