Anti-Human PPARGC1A Polyclonal Antibody (HP446014)

Name: Anti-Human PPARGC1A Polyclonal Antibody
Brand: abinScience
SKU: HP446014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HP446014

Description

Anti-Human PPARGC1A Polyclonal Antibody (HP446014) is a rabbit polyclonal antibody detecting PPARGC1A in ELISA, IHC, WB. Suitable for Human, Mouse, Rat, Bovine, and Pig.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PPARGC1A (Ser636-Arg798).

Target

LEM6, PGC1A, PPARGC1, Ligand effect modulator 6, PGC-1-alpha, PPAR-gamma coactivator 1-alpha, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, PPARGC-1-alpha, PPARGC1A, PGC1

Purification

Purified by antigen affinity column.

Accession

Q9UBK2

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A) is a ~91 kDa protein. Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Acts as a key regulator of gluconeogenesis: stimulates hepatic gluconeogenesis by increasing the expression of gluconeogenic enzymes, and acting together with FOXO1 to promote the fasting gluconeogenic program.

1. Knutti, D. et al. (2000) Molecular and cellular biology 20, 2411-22. PMID: 10713165

2. Dominy, JE. et al. (2010) Biochimica et biophysica acta 1804, 1676-83. PMID: 20005308

3. Shin, JH. et al. (2011) Cell 144, 689-702. PMID: 21376232

4. Mays, SG. et al. (2017) Molecular pharmacology 92, 1-11. PMID: 28363985

5. Seacrist, CD. et al. (2020) Structure (London, England : 1993) 28, 830-846.e9. PMID: 32433991

8. Cho, Y. et al. (2013) The Journal of biological chemistry 288, 25207-25218. PMID: 23836911

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human PPARGC1A polyclonal antibody at 1ug/mL on recombinant Human PPARGC1A (Catalog No. HP446012).

References

Datasheet

Anti-Human PPARGC1A Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human PPARGC1A Polyclonal Antibody (HP446014)

Overview

Images

References

Datasheet

SDS

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