Human PPP1R15A Recombinant Protein (N-GST & C-His) (HF783012)

Name: Human PPP1R15A Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HF783012
Price: 211 USD
Availability: InStock

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100ug
1mg

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Каталожный номер	HF783012
Описание	Recombinant Human PPP1R15A Protein, N-GST & C-His (HF783012) expressed in E. coli, spanning Ala571-Asn621. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	O75807
Длина белка	Ala571-Asn621
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	33.94 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	GADD34, Protein phosphatase 1 regulatory subunit 15A, PPP1R15A, Growth arrest and DNA damage-inducible protein GADD34, Myeloid differentiation primary response protein MyD116 homolog
Фон	Protein phosphatase 1 regulatory subunit 15A (PPP1R15A) is a ~73 kDa protein. Recruits the serine/threonine-protein phosphatase PPP1CA to prevents excessive phosphorylation of the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1. May promote apoptosis by inducing p53/TP53 phosphorylation on 'Ser-15'. Plays an essential role in autophagy by tuning translation during starvation, thus enabling lysosomal biogenesis and a sustained autophagic flux. Also acts a viral restriction factor by attenuating HIV-1 replication. 1. Choy, MS. et al. (2015) Cell reports 11, 1885-91. PMID: 26095357 2. Santos, CX. et al. (2016) The EMBO journal 35, 319-34. PMID: 26742780 3. Shi, W. et al. (2004) The Journal of cell biology 164, 291-300. PMID: 14718519 4. Yagi, A. et al. (2003) Journal of cellular biochemistry 90, 1242-9. PMID: 14635196 5. Gambardella, G. et al. (2020) Science advances 6. PMID: 32978159 6. Ishaq, M. et al. (2020) Virology 540, 119-131. PMID: 31778897
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human PPP1R15A Protein, N-GST & C-His

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Даташит

Human PPP1R15A Recombinant Protein (N-GST & C-His).pdf

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Formula

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C₁ (Stock Conc.)

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C₂ (Working Conc.)

V₂ (Working Vol.)

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