ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Homo sapiens (Human)
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
33.95 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия
PRKACA, PKACA, PKA C-alpha, cAMP-dependent protein kinase catalytic subunit alpha
Фон
cAMP-dependent protein kinase catalytic subunit alpha (PRKACA) is a ~40 kDa protein. Phosphorylates a large number of substrates in the cytoplasm and the nucleus. Phosphorylates CDC25B, ABL1, NFKB1, CLDN3, histone H1.4 (H1-4), PSMC5/RPT6, PJA2, RYR2, RORA, SOX9, UHRF1 and VASP. Regulates the abundance of compartmentalized pools of its regulatory subunits through phosphorylation of PJA2 which binds and ubiquitinates these subunits, leading to their subsequent proteolysis. RORA is activated by phosphorylation. Required for glucose-mediated adipogenic differentiation increase and osteogenic differentiation inhibition from osteoblasts.
1. Guan, H. et al. (2005) The Journal of biological chemistry 280, 9957-62. PMID: 15642694
2. D'Souza, T. et al. (2005) The Journal of biological chemistry 280, 26233-40. PMID: 15905176
3. Pasqualucci, L. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 395-400. PMID: 16387847
4. Xu, H. et al. (2008) Breast cancer research and treatment 107, 63-70. PMID: 17333334
5. Zhang, F. et al. (2007) The Journal of biological chemistry 282, 22460-71. PMID: 17565987
6. Xiao, B. et al. (2007) The Journal of biological chemistry 282, 30256-64. PMID: 17693412
7. Shirakawa, K. et al. (2008) Nature structural & molecular biology 15, 1184-91. PMID: 18836454
8. Wang, W. et al. (2010) Molecular and cellular biochemistry 338, 115-22. PMID: 19949837
9. Gambaryan, S. et al. (2010) The Journal of biological chemistry 285, 18352-63. PMID: 20356841
10. Murshid, A. et al. (2010) PloS one 5, e13830. PMID: 21085490
Примечание
For research use only.
Изображения
SDS-PAGE
SDS-PAGE for Recombinant Human PRKACA Protein, N-His
中文
English
한국어
日本語
Español
Français
Русский
