Human PTPN22 Recombinant Protein (N-His) (HB930012)

Name: Human PTPN22 Recombinant Protein (N-His)
Brand: abinScience
SKU: HB930012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HB930012
Description	Recombinant Human PTPN22 Protein, N-His (HB930012) expressed in E. coli, spanning Met1-Gly303. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q9Y2R2
Longueur de protéine	Met1-Gly303
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	37.79 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	PTPN8, Lymphoid phosphatase, PTPN22, PEST-domain phosphatase, LyP, Tyrosine-protein phosphatase non-receptor type 22, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, PEP
Contexte	Tyrosine-protein phosphatase non-receptor type 22 (PTPN22) is a ~91 kDa protein. Acts as a negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules. Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue. Dephosphorylates ZAP70 at its activating 'Tyr-493' residue. Dephosphorylates the immune system activator SKAP2. 1. Wu, J. et al. (2006) The Journal of biological chemistry 281, 11002-10. PMID: 16461343 2. Yu, X. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 19767-72. PMID: 18056643 3. Yu, X. et al. (2011) The Journal of biological chemistry 286, 30526-30534. PMID: 21719704 4. Wang, Y. et al. (2013) Immunity 39, 111-22. PMID: 23871208 5. Spalinger, MR. et al. (2013) PloS one 8, e72384. PMID: 23991106 6. Spalinger, MR. et al. (2016) The Journal of clinical investigation 126, 1783-800. PMID: 27043286
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human PTPN22 Protein, N-His

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Human PTPN22 Recombinant Protein (N-His).pdf

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