ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды
Homo sapiens (Human)
Характер
Recombinant
Уровень эндотоксинов
Please contact with the lab for this information.
Чистота
>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса
37.79 kDa
Форма
Lyophilized
Буфер хранения
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия
PTPN8, Lymphoid phosphatase, PTPN22, PEST-domain phosphatase, LyP, Tyrosine-protein phosphatase non-receptor type 22, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, PEP
Фон
Tyrosine-protein phosphatase non-receptor type 22 (PTPN22) is a ~91 kDa protein. Acts as a negative regulator of T-cell receptor (TCR) signaling by direct dephosphorylation of the Src family kinases LCK and FYN, ITAMs of the TCRz/CD3 complex, as well as ZAP70, VAV, VCP and other key signaling molecules. Associates with and probably dephosphorylates CBL. Dephosphorylates LCK at its activating 'Tyr-394' residue. Dephosphorylates ZAP70 at its activating 'Tyr-493' residue. Dephosphorylates the immune system activator SKAP2.
1. Wu, J. et al. (2006) The Journal of biological chemistry 281, 11002-10. PMID: 16461343
2. Yu, X. et al. (2007) Proceedings of the National Academy of Sciences of the United States of America 104, 19767-72. PMID: 18056643
3. Yu, X. et al. (2011) The Journal of biological chemistry 286, 30526-30534. PMID: 21719704
4. Wang, Y. et al. (2013) Immunity 39, 111-22. PMID: 23871208
5. Spalinger, MR. et al. (2013) PloS one 8, e72384. PMID: 23991106
6. Spalinger, MR. et al. (2016) The Journal of clinical investigation 126, 1783-800. PMID: 27043286
Примечание
For research use only.
Изображения
SDS-PAGE
SDS-PAGE for Recombinant Human PTPN22 Protein, N-His
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