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| カタログ番号 | HD611012 |
|---|---|
| 説明 |
Recombinant Human PUM1 Protein, N-His (HD611012) expressed in E. coli. Purity: >90% as determined by SDS-PAGE..
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q14671 |
| タンパク質長 | Ser830-Ile1186 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | HsPUM, KIAA0099, PUM1, PUMH1, Pumilio homolog 1, Pumilio-1 |
| 背景 | Pumilio homolog 1 is a ~126 kDa protein. Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle. 1. Gupta, YK. et al. (2008) Structure (London, England : 1993) 16, 549-57. PMID: 18328718 2. Lu, G. et al. (2011) Structure (London, England : 1993) 19, 361-7. PMID: 21397187 3. Filipovska, A. et al. (2011) Nature chemical biology 7, 425-7. PMID: 21572425 4. Dong, S. et al. (2011) The Journal of biological chemistry 286, 26732-42. PMID: 21653694 5. Van Etten, J. et al. (2012) The Journal of biological chemistry 287, 36370-83. PMID: 22955276 6. Galgano, A. et al. (2008) PloS one 3, e3164. PMID: 18776931 7. Kedde, M. et al. (2010) Nature cell biology 12, 1014-20. PMID: 20818387 8. Leibovich, L. et al. (2010) Silence 1, 17. PMID: 20860814 9. Miles, WO. et al. (2012) Genes & development 26, 356-68. PMID: 22345517 |
| 注意事項 | For research use only |
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