Anti-Human PUM1 Polyclonal Antibody (HD611014)

Name: Anti-Human PUM1 Polyclonal Antibody
Brand: abinScience
SKU: HD611014
Price: 168 USD
Availability: InStock

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Overview
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Datasheet
SDS

Overview

Catalog No.

HD611014

Description

Anti-Human PUM1 Polyclonal Antibody (HD611014) is a rabbit polyclonal antibody detecting PUM1. Suitable for Human and Mouse.

Highlights

●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human PUM1 (Ser830-Ile1186).

Target

HsPUM, KIAA0099, PUM1, PUMH1, Pumilio homolog 1, Pumilio-1

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q14671

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

Pumilio homolog 1 (PUM1) is a ~126 kDa protein. Sequence-specific RNA-binding protein that acts as a post-transcriptional repressor by binding the 3'-UTR of mRNA targets. Binds to an RNA consensus sequence, the Pumilio Response Element (PRE), 5'-UGUANAUA-3', that is related to the Nanos Response Element (NRE). Mediates post-transcriptional repression of transcripts via different mechanisms: acts via direct recruitment of the CCR4-POP2-NOT deadenylase leading to translational inhibition and mRNA degradation. Also mediates deadenylation-independent repression by promoting accessibility of miRNAs. Following growth factor stimulation, phosphorylated and binds to the 3'-UTR of CDKN1B/p27 mRNA, inducing a local conformational change that exposes miRNA-binding sites, promoting association of miR-221 and miR-222, efficient suppression of CDKN1B/p27 expression, and rapid entry to the cell cycle.

1. Gupta, YK. et al. (2008) Structure (London, England : 1993) 16, 549-57. PMID: 18328718

2. Lu, G. et al. (2011) Structure (London, England : 1993) 19, 361-7. PMID: 21397187

3. Filipovska, A. et al. (2011) Nature chemical biology 7, 425-7. PMID: 21572425

4. Dong, S. et al. (2011) The Journal of biological chemistry 286, 26732-42. PMID: 21653694

5. Van Etten, J. et al. (2012) The Journal of biological chemistry 287, 36370-83. PMID: 22955276

6. Galgano, A. et al. (2008) PloS one 3, e3164. PMID: 18776931

7. Kedde, M. et al. (2010) Nature cell biology 12, 1014-20. PMID: 20818387

8. Leibovich, L. et al. (2010) Silence 1, 17. PMID: 20860814

9. Miles, WO. et al. (2012) Genes & development 26, 356-68. PMID: 22345517

Note

For research use only

Images

References

Datasheet

Anti-Human PUM1 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human PUM1 Polyclonal Antibody (HD611014)

Overview

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References

Datasheet

SDS

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