Human RAB3IP Recombinant Protein (N-His) (HP867012)

Name: Human RAB3IP Recombinant Protein (N-His)
Brand: abinScience
SKU: HP867012
Price: 211 USD
Availability: InStock

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Aperçu

Numéro de catalogue	HP867012
Description	Recombinant Human RAB3IP Protein, N-His (HP867012) expressed in E. coli, spanning Asp314-Leu476. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Système d'expression	E. coli
Numéro d'accès	Q96QF0
Longueur de protéine	Asp314-Leu476
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Espèces	Homo sapiens (Human)
Nature	Recombinant
Niveau d'endotoxines	Please contact with the lab for this information.
Pureté	>90% as determined by SDS-PAGE.
Masse moléculaire prédite	21.26 kDa
Forme	Lyophilized
Tampon de stockage	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Reférez-vous aux informations spécifiques sur le tampon dans la copie papier du datasheet ou dans le COA spécifique au lot.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Expédition	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stabilité et stockage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Noms alternatifs	Rabin-3, Rab3A-interacting protein, Rab-3A-interacting protein, SSX2-interacting protein, RAB3IP, RABIN8
Contexte	Rab-3A-interacting protein (RAB3IP) is a ~53 kDa protein. Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis. 1. Hattula, K. et al. (2002) Molecular biology of the cell 13, 3268-80. PMID: 12221131 2. Steger, M. et al. (2016) eLife 5. PMID: 26824392 3. Yoshimura, S. et al. (2010) The Journal of cell biology 191, 367-81. PMID: 20937701 4. Bryant, DM. et al. (2010) Nature cell biology 12, 1035-45. PMID: 20890297 5. Wang, J. et al. (2015) Journal of cell science 128, 1375-85. PMID: 25673879 6. Walia, V. et al. (2019) Developmental cell 50, 229-246.e7. PMID: 31204173
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human RAB3IP Protein, N-His

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Human RAB3IP Recombinant Protein (N-His).pdf

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