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Anti-Human RAB3IP Polyclonal Antibody (HP867014)

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Overview
Catalog No.HP867014
Species reactivityHuman
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human RAB3IP (Asp314-Leu476).
Target Rabin-3, Rab3A-interacting protein, Rab-3A-interacting protein, SSX2-interacting protein, RAB3IP, RABIN8
Purification Purified by antigen affinity column.
Accession Q96QF0
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Rab-3A-interacting protein (RAB3IP) is a ~53 kDa protein. Guanine nucleotide exchange factor (GEF) which may activate RAB8A and RAB8B. Promotes the exchange of GDP to GTP, converting inactive GDP-bound Rab proteins into their active GTP-bound form. Mediates the release of GDP from RAB8A and RAB8B but not from RAB3A or RAB5. Modulates actin organization and promotes polarized transport of RAB8A-specific vesicles to the cell surface. Together with RAB11A, RAB8A, the exocyst complex, PARD3, PRKCI, ANXA2, CDC42 and DNMBP promotes transcytosis of PODXL to the apical membrane initiation sites (AMIS), apical surface formation and lumenogenesis.

1. Hattula, K. et al. (2002) Molecular biology of the cell 13, 3268-80. PMID: 12221131
2. Steger, M. et al. (2016) eLife 5. PMID: 26824392
3. Yoshimura, S. et al. (2010) The Journal of cell biology 191, 367-81. PMID: 20937701
4. Bryant, DM. et al. (2010) Nature cell biology 12, 1035-45. PMID: 20890297
5. Wang, J. et al. (2015) Journal of cell science 128, 1375-85. PMID: 25673879
6. Walia, V. et al. (2019) Developmental cell 50, 229-246.e7. PMID: 31204173
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
C₁ × V₁ = C₂ × V₂
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C₁ (Stock Conc.)
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-Human RAB3IP Polyclonal Antibody

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