中文
English
한국어
日本語
Español
Français
Русский
| カタログ番号 | HC389012 |
|---|---|
| 説明 |
Recombinant Human RAD51 Protein, N-His (HC389012) expressed in E. coli, spanning Val174-Asp339. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q06609 |
| タンパク質長 | Val174-Asp339 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 20.4 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | HsRAD51, RAD51A, RECA, hRAD51, RAD51, RAD51 homolog A, DNA repair protein RAD51 homolog 1 |
| 背景 | DNA repair protein RAD51 homolog 1 is a ~36 kDa protein. Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR). Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Recruited to resolve stalled replication forks during replication stress. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR. 1. Sigurdsson, S. et al. (2002) The Journal of biological chemistry 277, 42790-4. PMID: 12205100 2. Park, JY. et al. (2008) Nucleic acids research 36, 3226-34. PMID: 18417535 3. Islam, MN. et al. (2010) Molecular and cellular biology 30, 2460-72. PMID: 20231364 4. Schwendener, S. et al. (2010) The Journal of biological chemistry 285, 15739-45. PMID: 20348101 5. Yata, K. et al. (2012) Molecular cell 45, 371-83. PMID: 22325354 6. Wan, L. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10646-51. PMID: 23509288 7. Yuan, J. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10640-5. PMID: 23754376 8. Ameziane, N. et al. (2015) Nature communications 6, 8829. PMID: 26681308 9. Inano, S. et al. (2017) Molecular cell 66, 622-634.e8. PMID: 28575658 10. Brannvoll, A. et al. (2020) Cell reports 32, 107849. PMID: 32640219 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human RAD51 Protein, N-His
+86-027-65523339
中国武漢市深敦寺路666号C棟、武漢、430206
