Anti-RAD51 Polyclonal Antibody (HC389014)

Name: Anti-RAD51 Polyclonal Antibody
Brand: abinScience
SKU: HC389014
Price: 96 USD
Availability: InStock

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Overview

Catalog No.

HC389014

Description

Anti-RAD51 Polyclonal Antibody (HC389014) is a rabbit polyclonal antibody detecting RAD51 in ELISA, IHC, WB. Suitable for Bovine, Dog, Cricetulus griseus, Chicken, and Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RAD51 (Val174-Asp339).

Target

HsRAD51, RAD51A, RECA, hRAD51, RAD51, RAD51 homolog A, DNA repair protein RAD51 homolog 1

Purification

Purified by antigen affinity column.

Accession

Q06609

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

DNA repair protein RAD51 homolog 1 is a ~36 kDa protein. Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR). Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Recruited to resolve stalled replication forks during replication stress. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR.

1. Sigurdsson, S. et al. (2002) The Journal of biological chemistry 277, 42790-4. PMID: 12205100

2. Park, JY. et al. (2008) Nucleic acids research 36, 3226-34. PMID: 18417535

3. Islam, MN. et al. (2010) Molecular and cellular biology 30, 2460-72. PMID: 20231364

4. Schwendener, S. et al. (2010) The Journal of biological chemistry 285, 15739-45. PMID: 20348101

5. Yata, K. et al. (2012) Molecular cell 45, 371-83. PMID: 22325354

6. Wan, L. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10646-51. PMID: 23509288

7. Yuan, J. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10640-5. PMID: 23754376

8. Ameziane, N. et al. (2015) Nature communications 6, 8829. PMID: 26681308

9. Inano, S. et al. (2017) Molecular cell 66, 622-634.e8. PMID: 28575658

10. Brannvoll, A. et al. (2020) Cell reports 32, 107849. PMID: 32640219

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-RAD51 polyclonal antibody at 1ug/mL on various samples.
Lane 1: K562 cell lysate

References

Datasheet

Anti-RAD51 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

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Anti-RAD51 Polyclonal Antibody (HC389014)

Overview

Images

References

Datasheet

SDS

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