ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species
Homo sapiens (Human)
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Predicted molecular weight
20.4 kDa
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names
HsRAD51, RAD51A, RECA, hRAD51, RAD51, RAD51 homolog A, DNA repair protein RAD51 homolog 1
Background
DNA repair protein RAD51 homolog 1 is a ~36 kDa protein. Plays an important role in homologous strand exchange, a key step in DNA repair through homologous recombination (HR). Binds to single-stranded DNA in an ATP-dependent manner to form nucleoprotein filaments which are essential for the homology search and strand exchange. Catalyzes the recognition of homology and strand exchange between homologous DNA partners to form a joint molecule between a processed DNA break and the repair template. Recruited to resolve stalled replication forks during replication stress. Part of a PALB2-scaffolded HR complex containing BRCA2 and RAD51C and which is thought to play a role in DNA repair by HR.
1. Sigurdsson, S. et al. (2002) The Journal of biological chemistry 277, 42790-4. PMID: 12205100
2. Park, JY. et al. (2008) Nucleic acids research 36, 3226-34. PMID: 18417535
3. Islam, MN. et al. (2010) Molecular and cellular biology 30, 2460-72. PMID: 20231364
4. Schwendener, S. et al. (2010) The Journal of biological chemistry 285, 15739-45. PMID: 20348101
5. Yata, K. et al. (2012) Molecular cell 45, 371-83. PMID: 22325354
6. Wan, L. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10646-51. PMID: 23509288
7. Yuan, J. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 10640-5. PMID: 23754376
8. Ameziane, N. et al. (2015) Nature communications 6, 8829. PMID: 26681308
9. Inano, S. et al. (2017) Molecular cell 66, 622-634.e8. PMID: 28575658
10. Brannvoll, A. et al. (2020) Cell reports 32, 107849. PMID: 32640219
Note
For research use only.
Images
SDS-PAGE
SDS-PAGE for Recombinant Human RAD51 Protein, N-His
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