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| カタログ番号 | HX918012 |
|---|---|
| 説明 |
Recombinant Human RAN Protein, N-His (HX918012) expressed in E. coli, spanning Met1-Asp171. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | P62826 |
| タンパク質長 | Met1-Asp171 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 21.74 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | GTP-binding nuclear protein Ran, 3.6.5.-, Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein, RAN, ARA24, OK/SW-cl.81 |
| 背景 | GTP-binding nuclear protein Ran is a ~24 kDa protein. GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins. 1. Hsiao, PW. et al. (1999) The Journal of biological chemistry 274, 20229-34. PMID: 10400640 2. Yamashita, D. et al. (2007) The Journal of biological chemistry 282, 7563-75. PMID: 17209048 3. Rudack, T. et al. (2015) The Journal of biological chemistry 290, 24079-90. PMID: 26272610 4. Aksu, M. et al. (2016) Nature communications 7, 11952. PMID: 27306458 5. Melchior, F. et al. (1993) The Journal of cell biology 123, 1649-59. PMID: 8276887 6. Palacios, I. et al. (1996) The Journal of cell biology 133, 485-94. PMID: 8636225 7. Moroianu, J. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 7059-62. PMID: 8692944 8. Görlich, D. et al. (1996) The EMBO journal 15, 5584-94. PMID: 8896452 9. Izaurralde, E. et al. (1997) The EMBO journal 16, 6535-47. PMID: 9351834 10. Bischoff, FR. et al. (1997) FEBS letters 419, 249-54. PMID: 9428644 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human RAN Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
