Anti-Human RAN Polyclonal Antibody (HX918014)

Name: Anti-Human RAN Polyclonal Antibody
Brand: abinScience
SKU: HX918014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HX918014

Description

Anti-Human RAN Polyclonal Antibody (HX918014) is a rabbit polyclonal antibody detecting RAN in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human RAN (Met1-Asp171).

Target

GTP-binding nuclear protein Ran, 3.6.5.-, Androgen receptor-associated protein 24, GTPase Ran, Ras-like protein TC4, Ras-related nuclear protein, RAN, ARA24, OK/SW-cl.81

Purification

Purified by antigen affinity column.

Accession

P62826

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

GTP-binding nuclear protein Ran is a ~24 kDa protein. GTPase involved in nucleocytoplasmic transport, participating both to the import and the export from the nucleus of proteins and RNAs. Switches between a cytoplasmic GDP- and a nuclear GTP-bound state by nucleotide exchange and GTP hydrolysis. Nuclear import receptors such as importin beta bind their substrates only in the absence of GTP-bound RAN and release them upon direct interaction with GTP-bound RAN, while export receptors behave in the opposite way. Thereby, RAN controls cargo loading and release by transport receptors in the proper compartment and ensures the directionality of the transport. Interaction with RANBP1 induces a conformation change in the complex formed by XPO1 and RAN that triggers the release of the nuclear export signal of cargo proteins.

1. Hsiao, PW. et al. (1999) The Journal of biological chemistry 274, 20229-34. PMID: 10400640

2. Yamashita, D. et al. (2007) The Journal of biological chemistry 282, 7563-75. PMID: 17209048

3. Rudack, T. et al. (2015) The Journal of biological chemistry 290, 24079-90. PMID: 26272610

4. Aksu, M. et al. (2016) Nature communications 7, 11952. PMID: 27306458

5. Melchior, F. et al. (1993) The Journal of cell biology 123, 1649-59. PMID: 8276887

6. Palacios, I. et al. (1996) The Journal of cell biology 133, 485-94. PMID: 8636225

7. Moroianu, J. et al. (1996) Proceedings of the National Academy of Sciences of the United States of America 93, 7059-62. PMID: 8692944

8. Görlich, D. et al. (1996) The EMBO journal 15, 5584-94. PMID: 8896452

9. Izaurralde, E. et al. (1997) The EMBO journal 16, 6535-47. PMID: 9351834

10. Bischoff, FR. et al. (1997) FEBS letters 419, 249-54. PMID: 9428644

Note

For research use only.

Images

References

Datasheet

Anti-Human RAN Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-Human RAN Polyclonal Antibody (HX918014)

Overview

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Datasheet

SDS

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