Human RAP2A Recombinant Protein (N-His) (HY078012)

Name: Human RAP2A Recombinant Protein (N-His)
Brand: abinScience
SKU: HY078012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Обзор

Каталожный номер	HY078012
Описание	Recombinant Human RAP2A Protein, N-His (HY078012) expressed in E. coli, spanning Met1-Gln183. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	P10114
Длина белка	Met1-Gln183
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	22.78 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	Ras-related protein Rap-2a, 3.6.5.2, RbBP-30, RAP2A
Фон	Ras-related protein Rap-2a (RAP2A) is a ~20 kDa protein. Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and regulates cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading. 1. Machida, N. et al. (2004) The Journal of biological chemistry 279, 15711-4. PMID: 14966141 2. Taira, K. et al. (2004) The Journal of biological chemistry 279, 49488-96. PMID: 15342639 3. Mittal, V. et al. (2006) The Biochemical journal 394, 309-15. PMID: 16246175 4. Greco, F. et al. (2006) Biochimica et biophysica acta 1763, 330-5. PMID: 16540189 5. Nonaka, H. et al. (2008) Biochemical and biophysical research communications 377, 573-578. PMID: 18930710 6. Kawabe, H. et al. (2010) Neuron 65, 358-72. PMID: 20159449 7. Duncan, ED. et al. (2022) The Journal of cell biology 221. PMID: 35293963
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human RAP2A Protein, N-His

Ссылки

Даташит

Human RAP2A Recombinant Protein (N-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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