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Anti-Human RAP2A Polyclonal Antibody (HY078014)

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Overview
Catalog No.HY078014
Description
Anti-Human RAP2A Polyclonal Antibody (HY078014) is a rabbit polyclonal antibody detecting RAP2A in ELISA, IHC, WB. Suitable for Human.
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityHuman, Mouse, Rat
ApplicationsELISA, IHC, WB
Host speciesRabbit
ClonalityPolyclonal
IsotypeIgG
Immunogen E. coli - derived recombinant Human RAP2A (Met1-Gln183).
Target Ras-related protein Rap-2a, 3.6.5.2, RbBP-30, RAP2A
Purification Purified by antigen affinity column.
Accession P10114
Form Liquid
Storage buffer 0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:5000-1:20000
IHC 1:50-1:500
WB 1:500-1:2000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background

Ras-related protein Rap-2a (RAP2A) is a ~20 kDa protein. Small GTP-binding protein which cycles between a GDP-bound inactive and a GTP-bound active form. In its active form interacts with and regulates several effectors including MAP4K4, MINK1 and TNIK. Part of a signaling complex composed of NEDD4, RAP2A and TNIK which regulates neuronal dendrite extension and arborization during development. More generally, it is part of several signaling cascades and regulates cytoskeletal rearrangements, cell migration, cell adhesion and cell spreading.

1. Machida, N. et al. (2004) The Journal of biological chemistry 279, 15711-4. PMID: 14966141
2. Taira, K. et al. (2004) The Journal of biological chemistry 279, 49488-96. PMID: 15342639
3. Mittal, V. et al. (2006) The Biochemical journal 394, 309-15. PMID: 16246175
4. Greco, F. et al. (2006) Biochimica et biophysica acta 1763, 330-5. PMID: 16540189
5. Nonaka, H. et al. (2008) Biochemical and biophysical research communications 377, 573-578. PMID: 18930710
6. Kawabe, H. et al. (2010) Neuron 65, 358-72. PMID: 20159449
7. Duncan, ED. et al. (2022) The Journal of cell biology 221. PMID: 35293963
Note For research use only.
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Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
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Formula
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V₁ (Stock Vol.)
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C₂ (Working Conc.)
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Anti-Human RAP2A Polyclonal Antibody

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