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| カタログ番号 | HP601012 |
|---|---|
| 説明 |
Recombinant Human RICTOR Protein, N-His-SUMO & C-Strep (HP601012) expressed in E. coli, spanning Arg32-Glu140. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q6R327 |
| タンパク質長 | Arg32-Glu140 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 26.15 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | KIAA1999, Rapamycin-insensitive companion of mTOR, hAVO3, RICTOR, AVO3 homolog |
| 背景 | Rapamycin-insensitive companion of mTOR (RICTOR) is a ~192 kDa protein. Component of the mechanistic target of rapamycin complex 2 (mTORC2), which transduces signals from growth factors to pathways involved in proliferation, cytoskeletal organization, lipogenesis and anabolic output. In response to growth factors, mTORC2 phosphorylates and activates AGC protein kinase family members, including AKT (AKT1, AKT2 and AKT3), PKC (PRKCA, PRKCB and PRKCE) and SGK1. In contrast to mTORC1, mTORC2 is nutrient-insensitive. Within the mTORC2 complex, RICTOR probably acts as a molecular adapter. RICTOR is responsible for the FKBP12-rapamycin-insensitivity of mTORC2. 1. Sarbassov, DD. et al. (2004) Current biology : CB 14, 1296-302. PMID: 15268862 2. Sarbassov, DD. et al. (2005) Science (New York, N.Y.) 307, 1098-101. PMID: 15718470 3. Dibble, CC. et al. (2009) Molecular and cellular biology 29, 5657-70. PMID: 19720745 4. Julien, LA. et al. (2010) Molecular and cellular biology 30, 908-21. PMID: 19995915 5. Chen, CH. et al. (2011) Science signaling 4, ra10. PMID: 21343617 6. Scaiola, A. et al. (2020) Science advances 6. PMID: 33158864 7. Zhuang, L. et al. (2022) Cell biology international 46, 1693-1703. PMID: 35904232 8. Yu, Z. et al. (2022) The Journal of biological chemistry 298, 102288. PMID: 35926713 9. Treins, C. et al. (2010) Oncogene 29, 1003-16. PMID: 19935711 10. Jacinto, E. et al. (2004) Nature cell biology 6, 1122-8. PMID: 15467718 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human RICTOR Protein, N-His-SUMO & C-Strep
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