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| カタログ番号 | HT159012 |
|---|---|
| 説明 |
Recombinant Human RIOK3 Protein, N-His (HT159012) expressed in E. coli, spanning Ser247-Glu519. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | O14730 |
| タンパク質長 | Ser247-Glu519 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 33.69 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Serine/threonine-protein kinase RIO3, SUDD, sudD homolog, RIO kinase 3, RIOK3 |
| 背景 | Serine/threonine-protein kinase RIO3 (RIOK3) is a ~59 kDa protein. Serine/threonine-protein kinase involved in a ribosome quality control that takes place when ribosomes have stalled, leading to 18S non-functional rRNA decay and degradation of the 40S ribosomal subunit. Acts downstream of RNF10: specifically recognizes and binds RPS2/us5 and RPS3/us3 monoubiquitinated by RNF10, promoting degradation of the 40S ribosomal subunit in a kinase-dependent manner. The RNF10-RIOK3 ribosome quality control takes place in response to ribosome subunit imbalance or downstream the EIF2AK4/GCN2-mediated integrated stress response (ISR). Also involved in regulation of type I interferon (IFN)-dependent immune response, possibly by acting as an adapter protein essential for the recruitment of TBK1 to IRF3. Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling. 1. Coria, AR. et al. (2025) Molecular cell 85, 787-801.e8. PMID: 39947182 2. Huang, Z. et al. (2025) Molecular cell 85, 802-814.e12. PMID: 39947183 3. Ford, PW. et al. (2025) Cell reports 44, 115371. PMID: 40022732 4. Feng, J. et al. (2014) Journal of virology 88, 7987-97. PMID: 24807708 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human RIOK3 Protein, N-His
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