Anti-Human RIOK3 Polyclonal Antibody (HT159014)

Name: Anti-Human RIOK3 Polyclonal Antibody
Brand: abinScience
SKU: HT159014
Price: 96 USD
Availability: InStock

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Overview
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References
Datasheet
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Overview

Catalog No.

HT159014

Species reactivity

Human, Mouse

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human RIOK3 (Ser247-Glu519).

Target

Serine/threonine-protein kinase RIO3, SUDD, sudD homolog, RIO kinase 3, RIOK3

Purification

Purified by antigen affinity column.

Accession

O14730

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Serine/threonine-protein kinase RIO3 (RIOK3) is a ~59 kDa protein. Serine/threonine-protein kinase involved in a ribosome quality control that takes place when ribosomes have stalled, leading to 18S non-functional rRNA decay and degradation of the 40S ribosomal subunit. Acts downstream of RNF10: specifically recognizes and binds RPS2/us5 and RPS3/us3 monoubiquitinated by RNF10, promoting degradation of the 40S ribosomal subunit in a kinase-dependent manner. The RNF10-RIOK3 ribosome quality control takes place in response to ribosome subunit imbalance or downstream the EIF2AK4/GCN2-mediated integrated stress response (ISR). Also involved in regulation of type I interferon (IFN)-dependent immune response, possibly by acting as an adapter protein essential for the recruitment of TBK1 to IRF3. Phosphorylates IFIH1 on 'Ser-828' interfering with IFIH1 filament assembly on long dsRNA and resulting in attenuated IFIH1-signaling.

1. Coria, AR. et al. (2025) Molecular cell 85, 787-801.e8. PMID: 39947182

2. Huang, Z. et al. (2025) Molecular cell 85, 802-814.e12. PMID: 39947183

3. Ford, PW. et al. (2025) Cell reports 44, 115371. PMID: 40022732

4. Feng, J. et al. (2014) Journal of virology 88, 7987-97. PMID: 24807708

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-RIOK3 polyclonal antibody at 1µg/ml on various samples.
Lane 1: NIH3T3 cell lysate
Lane 2: Jurkat cell lysate
Lane 3: K562 cell lysate
Lane 4: Mouse testis lysate
Lane 5: Rat testis lysate

References

Datasheet

Anti-Human RIOK3 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human RIOK3 Polyclonal Antibody (HT159014)

Overview

Images

References

Datasheet

SDS

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