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Human RNASE7 Recombinant Protein (C-His) (HV262011)

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Vista general
Número de catálogoHV262011
Descripción
Recombinant Human RNASE7 Protein, C-His (HV262011) expressed in Yeast Cells, spanning Lys29-Leu156. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresiónYeast cells
Número de accesoQ9H1E1
Longitud de proteínaLys29-Leu156
AplicacionesELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
EspeciesHomo sapiens (Human)
Naturaleza Recombinant
Nivel de endotoxinas Please contact with the lab for this information.
Pureza >90% as determined by SDS-PAGE.
Peso molecular predicho 15.82 kDa
Forma Lyophilized
Buffer de almacenamiento Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.

Reconstitución Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Envío In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativosEC:3.1.27.-, RNASE7, RNase 7, Ribonuclease 7, SAP-2, Skin-derived antimicrobial protein 2
Fondo

Ribonuclease 7 is a ~17 kDa protein. Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms including uropathogenic E.coli (UPEC), and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium. Causes loss of bacterial membrane integrity. Probably contributes to urinary tract sterility. Bactericidal activity is independent of RNase activity.

1. Harder, J. et al. (2002) The Journal of biological chemistry 277, 46779-84. PMID: 12244054
2. Zhang, J. et al. (2003) Nucleic acids research 31, 602-7. PMID: 12527768
3. Huang, YC. et al. (2007) The Journal of biological chemistry 282, 4626-4633. PMID: 17150966
4. Becknell, B. et al. (2015) Kidney international 87, 151-61. PMID: 25075772
5. Bender, K. et al. (2021) American journal of physiology. Renal physiology 320, F972-F983. PMID: 33818125
Nota For research use only
Imágenes
Referencias
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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