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Human RNASE7 Recombinant Protein (C-His) (HV262011)

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概要
カタログ番号HV262011
説明
Recombinant Human RNASE7 Protein, C-His (HV262011) expressed in Yeast Cells, spanning Lys29-Leu156. Purity: >90% by SDS-PAGE.
Highlights
  • ●His-Tagged — N-terminal 6×His tag for IMAC purification.
  • ●High Purity — >90% purity verified by SDS-PAGE.
発現システムYeast cells
アクセッション番号Q9H1E1
タンパク質長Lys29-Leu156
アプリケーションELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
種Homo sapiens (Human)
性質 Recombinant
エンドトキシンレベル Please contact with the lab for this information.
純度 >90% as determined by SDS-PAGE.
予測分子量 15.82 kDa
形態 Lyophilized
保存バッファー Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol.

データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。

再構成 Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
発送 In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
安定性と保存 Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
別名EC:3.1.27.-, RNASE7, RNase 7, Ribonuclease 7, SAP-2, Skin-derived antimicrobial protein 2
背景

Ribonuclease 7 is a ~17 kDa protein. Exhibits a potent RNase activity. Has broad-spectrum antimicrobial activity against many pathogenic microorganisms including uropathogenic E.coli (UPEC), and remarkably potent activity (lethal dose of 90% < 30 nM) against a vancomycin resistant Enterococcus faecium. Causes loss of bacterial membrane integrity. Probably contributes to urinary tract sterility. Bactericidal activity is independent of RNase activity.

1. Harder, J. et al. (2002) The Journal of biological chemistry 277, 46779-84. PMID: 12244054
2. Zhang, J. et al. (2003) Nucleic acids research 31, 602-7. PMID: 12527768
3. Huang, YC. et al. (2007) The Journal of biological chemistry 282, 4626-4633. PMID: 17150966
4. Becknell, B. et al. (2015) Kidney international 87, 151-61. PMID: 25075772
5. Bender, K. et al. (2021) American journal of physiology. Renal physiology 320, F972-F983. PMID: 33818125
注意事項 For research use only
イメージ
参考文献
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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