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| カタログ番号 | HA051012 |
|---|---|
| 説明 |
Recombinant Human RNF2 Protein, N-His (HA051012) expressed in E. coli, spanning Glu225-Lys336. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q99496 |
| タンパク質長 | Glu225-Lys336 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 15.13 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | Huntingtin-interacting protein 2-interacting protein 3, RING-type E3 ubiquitin transferase RING2, E3 ubiquitin-protein ligase RING2, HIP2-interacting protein 3, DING, RING finger protein 1B, BAP1, Protein DinG, RING1B, RING finger protein 2, HIPI3, RNF2, RING finger protein BAP-1, RING1b |
| 背景 | E3 ubiquitin-protein ligase RING2 (RNF2) is a ~37 kDa protein. E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation. H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. 1. Wang, H. et al. (2004) Nature 431, 873-8. PMID: 15386022 2. Cao, R. et al. (2005) Molecular cell 20, 845-54. PMID: 16359901 3. Bentley, ML. et al. (2011) The EMBO journal 30, 3285-97. PMID: 21772249 4. McGinty, RK. et al. (2014) Nature 514, 591-6. PMID: 25355358 5. Gao, Z. et al. (2014) Nature 516, 349-54. PMID: 25519132 6. Taherbhoy, AM. et al. (2015) Nature communications 6, 7621. PMID: 26151332 7. Luo, X. et al. (2021) Human molecular genetics 30, 1283-1292. PMID: 33864376 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human RNF2 Protein, N-His
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