Anti-Human RNF2 Polyclonal Antibody (HA051014)

Name: Anti-Human RNF2 Polyclonal Antibody
Brand: abinScience
SKU: HA051014
Price: 168 USD
Availability: InStock

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Overview

Catalog No.

HA051014

Description

Anti-Human RNF2 Polyclonal Antibody (HA051014) is a rabbit polyclonal antibody detecting RNF2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human, Mouse, Rat

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human RNF2 (Glu225-Lys336).

Target

Huntingtin-interacting protein 2-interacting protein 3, RING-type E3 ubiquitin transferase RING2, E3 ubiquitin-protein ligase RING2, HIP2-interacting protein 3, DING, RING finger protein 1B, BAP1, Protein DinG, RING1B, RING finger protein 2, HIPI3, RNF2, RING finger protein BAP-1, RING1b

Purification

Purified by antigen affinity column.

Accession

Q99496

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

E3 ubiquitin-protein ligase RING2 (RNF2) is a ~37 kDa protein. E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation. H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility.

1. Wang, H. et al. (2004) Nature 431, 873-8. PMID: 15386022

2. Cao, R. et al. (2005) Molecular cell 20, 845-54. PMID: 16359901

3. Bentley, ML. et al. (2011) The EMBO journal 30, 3285-97. PMID: 21772249

4. McGinty, RK. et al. (2014) Nature 514, 591-6. PMID: 25355358

5. Gao, Z. et al. (2014) Nature 516, 349-54. PMID: 25519132

6. Taherbhoy, AM. et al. (2015) Nature communications 6, 7621. PMID: 26151332

7. Luo, X. et al. (2021) Human molecular genetics 30, 1283-1292. PMID: 33864376

Note

For research use only.

Images

References

Datasheet

Anti-Human RNF2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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Anti-Human RNF2 Polyclonal Antibody (HA051014)

Overview

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References

Datasheet

SDS

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