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| カタログ番号 | HC711012 |
|---|---|
| 説明 |
Recombinant Human RNF31 Protein, N-His (HC711012) expressed in E. coli, spanning Met1-Lys181. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q96EP0 |
| タンパク質長 | Met1-Lys181 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 22.74 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | E3 ubiquitin-protein ligase RNF31, EC:2.3.2.31, HOIL-1-interacting protein, HOIP, RING finger protein 31, RING-type E3 ubiquitin transferase RNF31, RNF31, ZIBRA, Zinc in-between-RING-finger ubiquitin-associated domain protein |
| 背景 | E3 ubiquitin-protein ligase RNF31 is a ~119 kDa protein. E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria. 1. Kirisako, T. et al. (2006) The EMBO journal 25, 4877-87. PMID: 17006537 2. Tokunaga, F. et al. (2009) Nature cell biology 11, 123-32. PMID: 19136968 3. Haas, TL. et al. (2009) Molecular cell 36, 831-44. PMID: 20005846 4. Gerlach, B. et al. (2011) Nature 471, 591-6. PMID: 21455173 5. Tokunaga, F. et al. (2011) Nature 471, 633-6. PMID: 21455180 6. Ikeda, F. et al. (2011) Nature 471, 637-41. PMID: 21455181 7. Smit, JJ. et al. (2012) The EMBO journal 31, 3833-44. PMID: 22863777 8. Goto, E. et al. (2017) Biochemical and biophysical research communications 485, 152-159. PMID: 28189684 9. Noad, J. et al. (2017) Nature microbiology 2, 17063. PMID: 28481331 10. Schlicher, L. et al. (2016) EMBO reports 17, 1485-1497. PMID: 27458237 |
| 注意事項 | For research use only |
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