Anti-Human RNF31 Polyclonal Antibody (HC711014)

Name: Anti-Human RNF31 Polyclonal Antibody
Brand: abinScience
SKU: HC711014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HC711014

Description

Anti-Human RNF31 Polyclonal Antibody (HC711014) is a rabbit polyclonal antibody detecting RNF31. Suitable for Human.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RNF31 (Met1-Lys181).

Target

E3 ubiquitin-protein ligase RNF31, EC:2.3.2.31, HOIL-1-interacting protein, HOIP, RING finger protein 31, RING-type E3 ubiquitin transferase RNF31, RNF31, ZIBRA, Zinc in-between-RING-finger ubiquitin-associated domain protein

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

Q96EP0

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Background

E3 ubiquitin-protein ligase RNF31 is a ~119 kDa protein. E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria.

1. Kirisako, T. et al. (2006) The EMBO journal 25, 4877-87. PMID: 17006537

2. Tokunaga, F. et al. (2009) Nature cell biology 11, 123-32. PMID: 19136968

3. Haas, TL. et al. (2009) Molecular cell 36, 831-44. PMID: 20005846

4. Gerlach, B. et al. (2011) Nature 471, 591-6. PMID: 21455173

5. Tokunaga, F. et al. (2011) Nature 471, 633-6. PMID: 21455180

6. Ikeda, F. et al. (2011) Nature 471, 637-41. PMID: 21455181

7. Smit, JJ. et al. (2012) The EMBO journal 31, 3833-44. PMID: 22863777

8. Goto, E. et al. (2017) Biochemical and biophysical research communications 485, 152-159. PMID: 28189684

9. Noad, J. et al. (2017) Nature microbiology 2, 17063. PMID: 28481331

10. Schlicher, L. et al. (2016) EMBO reports 17, 1485-1497. PMID: 27458237

Note

For research use only

Images

References

Datasheet

Anti-Human RNF31 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Mass

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Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-Human RNF31 Polyclonal Antibody (HC711014)

Overview

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Datasheet

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