Human RNF31 Recombinant Protein (N-His) (HC711012)

Name: Human RNF31 Recombinant Protein (N-His)
Brand: abinScience
SKU: HC711012
Price: 211 USD
Availability: InStock

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Catalog No.	HC711012
Description	Recombinant Human RNF31 Protein, N-His (HC711012) expressed in E. coli, spanning Met1-Lys181. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	Q96EP0
Protein length	Met1-Lys181
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	22.74 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	E3 ubiquitin-protein ligase RNF31, EC:2.3.2.31, HOIL-1-interacting protein, HOIP, RING finger protein 31, RING-type E3 ubiquitin transferase RNF31, RNF31, ZIBRA, Zinc in-between-RING-finger ubiquitin-associated domain protein
Background	E3 ubiquitin-protein ligase RNF31 is a ~119 kDa protein. E3 ubiquitin-protein ligase component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. The LUBAC complex is also involved in innate immunity by conjugating linear polyubiquitin chains at the surface of bacteria invading the cytosol to form the ubiquitin coat surrounding bacteria. 1. Kirisako, T. et al. (2006) The EMBO journal 25, 4877-87. PMID: 17006537 2. Tokunaga, F. et al. (2009) Nature cell biology 11, 123-32. PMID: 19136968 3. Haas, TL. et al. (2009) Molecular cell 36, 831-44. PMID: 20005846 4. Gerlach, B. et al. (2011) Nature 471, 591-6. PMID: 21455173 5. Tokunaga, F. et al. (2011) Nature 471, 633-6. PMID: 21455180 6. Ikeda, F. et al. (2011) Nature 471, 637-41. PMID: 21455181 7. Smit, JJ. et al. (2012) The EMBO journal 31, 3833-44. PMID: 22863777 8. Goto, E. et al. (2017) Biochemical and biophysical research communications 485, 152-159. PMID: 28189684 9. Noad, J. et al. (2017) Nature microbiology 2, 17063. PMID: 28481331 10. Schlicher, L. et al. (2016) EMBO reports 17, 1485-1497. PMID: 27458237
Note	For research use only

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Human RNF31 Recombinant Protein (N-His).pdf

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Human RNF31 Recombinant Protein (N-His) (HC711012)

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