

| Каталожный номер | HY509012 |
|---|---|
| Описание |
Recombinant Human RNH1 Protein, N-His (HY509012) expressed in E. coli, spanning Asp218-Glu444. Purity: >90% by SDS-PAGE.
Highlights
|
| Система экспрессии | E. coli |
| Номер доступа | P13489 |
| Длина белка | Asp218-Glu444 |
| Применения | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| Виды | Homo sapiens (Human) |
| Характер | Recombinant |
| Уровень эндотоксинов | Please contact with the lab for this information. |
| Чистота | >90% as determined by SDS-PAGE. |
| Прогнозируемая молекулярная масса | 26.87 kDa |
| Форма | Lyophilized |
| Буфер хранения | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии. |
| Реконституция | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| Доставка | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| Устойчивость и хранение | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| Альтернативные названия | RNH, Placental ribonuclease inhibitor, PRI, Placental RNase inhibitor, Ribonuclease/angiogenin inhibitor 1, RAI, RNH1, Ribonuclease inhibitor |
| Фон | Ribonuclease inhibitor (RNH1) is a ~49 kDa protein. Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and angiogenin (ANG). May play a role in redox homeostasis. Required to inhibit the cytotoxic tRNA ribonuclease activity of ANG in the cytoplasm in absence of stress. Relocates to the nucleus in response to stress, relieving inhibition of ANG in the cytoplasm, and inhibiting the angiogenic activity of ANG in the nucleus. 1. Teufel, DP. et al. (2003) Biochemistry 42, 1451-9. PMID: 12578357 2. Huang, GH. et al. (2003) Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica 35, 960-3. PMID: 14515218 3. Lee, FS. et al. (1988) Biochemistry 27, 8545-53. PMID: 3219362 4. Schneider, R. et al. (1988) The EMBO journal 7, 4151-6. PMID: 3243277 5. Shapiro, R. et al. (1987) Proceedings of the National Academy of Sciences of the United States of America 84, 2238-41. PMID: 3470787 6. Chen, CZ. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 1761-6. PMID: 9050852 7. Monti, DM. et al. (2007) FEBS letters 581, 930-4. PMID: 17292889 8. Pizzo, E. et al. (2013) Journal of cell science 126, 4308-19. PMID: 23843625 9. Bai, R. et al. (2020) The EMBO journal 39, e103325. PMID: 32510170 |
| Примечание | For research use only. |

SDS-PAGE for Recombinant Human RNH1 Protein, N-His
Свяжитесь с нами для индивидуальных предложений, оптовых запросов и других вопросов.
Почта: [email protected]


+86-027-65523339
Building C, No. 666, Shen Dun Si Lu, Wuhan, 430206, China
中文
English
한국어
日本語
Español
Français
Русский