Anti-Human RNH1 Polyclonal Antibody (HY509014)

Name: Anti-Human RNH1 Polyclonal Antibody
Brand: abinScience
SKU: HY509014
Price: 96 USD
Availability: InStock

Datasheet Share product Copy info

Price(USD):

Spec:

20ug
50ug
100ug

Number:

Get quote

Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY509014

Description

Anti-Human RNH1 Polyclonal Antibody (HY509014) is a rabbit polyclonal antibody detecting RNH1 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RNH1 (Asp218-Glu444).

Target

Ribonuclease inhibitor, Placental ribonuclease inhibitor, Placental RNase inhibitor, Ribonuclease/angiogenin inhibitor 1, RAI, RNH1, PRI, RNH

Endotoxin level

Please contact with the lab for this information.

Purification

Purified by antigen affinity column.

Accession

P13489

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Ribonuclease inhibitor (RNH1) is a ~49 kDa protein. Ribonuclease inhibitor which inhibits RNASE1, RNASE2 and angiogenin (ANG). May play a role in redox homeostasis. Required to inhibit the cytotoxic tRNA ribonuclease activity of ANG in the cytoplasm in absence of stress. Relocates to the nucleus in response to stress, relieving inhibition of ANG in the cytoplasm, and inhibiting the angiogenic activity of ANG in the nucleus.

1. Teufel, DP. et al. (2003) Biochemistry 42, 1451-9. PMID: 12578357

2. Huang, GH. et al. (2003) Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica 35, 960-3. PMID: 14515218

3. Lee, FS. et al. (1988) Biochemistry 27, 8545-53. PMID: 3219362

4. Schneider, R. et al. (1988) The EMBO journal 7, 4151-6. PMID: 3243277

5. Shapiro, R. et al. (1987) Proceedings of the National Academy of Sciences of the United States of America 84, 2238-41. PMID: 3470787

6. Chen, CZ. et al. (1997) Proceedings of the National Academy of Sciences of the United States of America 94, 1761-6. PMID: 9050852

7. Monti, DM. et al. (2007) FEBS letters 581, 930-4. PMID: 17292889

8. Pizzo, E. et al. (2013) Journal of cell science 126, 4308-19. PMID: 23843625

9. Bai, R. et al. (2020) The EMBO journal 39, e103325. PMID: 32510170

Note

For research use only

Images

Western blot

Western blot analysis was performed using anti-RNH1 polyclonal antibody at 1ug/mL on various samples.
Lane 1: HepG2 cell lysate
Lane 2: LO2 cell lysate
Lane 3: Jurkat cell lysate
Lane 4: U87 cell lysate
Lane 5: Raji cell lysate

References

Datasheet

Anti-Human RNH1 Polyclonal Antibody.pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human RNH1 Polyclonal Antibody (HY509014)

Overview

Images

References

Datasheet

SDS

Quick access

Contact Us