Human RSPO3 Recombinant Protein (N-His) (HV514012)

Name: Human RSPO3 Recombinant Protein (N-His)
Brand: abinScience
SKU: HV514012
Price: 211 USD
Availability: InStock

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50ug
100ug
1mg

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Каталожный номер	HV514012
Описание	Recombinant Human RSPO3 Protein, N-His (HV514012) expressed in E. coli, spanning Gly27-His272. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Система экспрессии	E. coli
Номер доступа	Q9BXY4
Длина белка	Gly27-His272
Применения	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Виды	Homo sapiens (Human)
Характер	Recombinant
Уровень эндотоксинов	Please contact with the lab for this information.
Чистота	>90% as determined by SDS-PAGE.
Прогнозируемая молекулярная масса	30.09 kDa
Форма	Lyophilized
Буфер хранения	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Пожалуйста, обратитесь к конкретной информации о буфере в печатной версии даташита или в индивидуальном сертификате качества (COA) для партии.
Реконституция	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Доставка	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Устойчивость и хранение	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Альтернативные названия	RSPO3, hPWTSR, hRspo3, PWTSR, R-spondin-3, Thrombospondin type-1 domain-containing protein 2, Protein with TSP type-1 repeat, Roof plate-specific spondin-3, THSD2
Фон	R-spondin-3 (RSPO3) is a ~30 kDa protein. Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. 1. de Lau, W. et al. (2011) Nature 476, 293-7. PMID: 21727895 2. Glinka, A. et al. (2011) EMBO reports 12, 1055-61. PMID: 21909076 3. Gong, X. et al. (2012) PloS one 7, e37137. PMID: 22615920 4. Szenker-Ravi, E. et al. (2018) Nature 557, 564-569. PMID: 29769720
Примечание	For research use only.

Изображения

SDS-PAGE

SDS-PAGE for Recombinant Human RSPO3 Protein, N-His

Ссылки

Даташит

Human RSPO3 Recombinant Protein (N-His).pdf

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Formula

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C₁ (Stock Conc.)

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