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| カタログ番号 | HP214012 |
|---|---|
| 説明 |
Recombinant Human SETD2 Protein, N-His (HP214012) expressed in E. coli, spanning Leu1444-Val1834. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9BYW2 |
| タンパク質長 | Leu1444-Val1834 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 47.38 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | EC:2.1.1.-, EC:2.1.1.359, HIF-1, HIF1, HIP-1, HYPB, Histone-lysine N-methyltransferase SETD2, Huntingtin yeast partner B, Huntingtin-interacting protein 1, Huntingtin-interacting protein B, KIAA1732, KMT3A, Lysine N-methyltransferase 3A, Protein-lysine N-methyltransferase SETD2, SET domain-containing protein 2, SET2, SETD2, hSET2, p231HBP |
| 背景 | Histone-lysine N-methyltransferase SETD2 is a ~287 kDa protein. Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate. It is capable of trimethylating unmethylated H3K36 (H3K36me0) in vitro. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction. 1. Sun, XJ. et al. (2005) The Journal of biological chemistry 280, 35261-71. PMID: 16118227 2. Yoh, SM. et al. (2008) Genes & development 22, 3422-34. PMID: 19141475 3. Hahn, MA. et al. (2011) PloS one 6, e18844. PMID: 21526191 4. de Almeida, SF. et al. (2011) Nature structural & molecular biology 18, 977-83. PMID: 21792193 5. Zheng, W. et al. (2012) Journal of the American Chemical Society 134, 18004-14. PMID: 23043551 6. Yang, S. et al. (2016) Genes & development 30, 1611-6. PMID: 27474439 7. Yuan, W. et al. (2009) The Journal of biological chemistry 284, 15701-7. PMID: 19332550 8. Carvalho, S. et al. (2013) Nucleic acids research 41, 2881-93. PMID: 23325844 9. Li, F. et al. (2013) Cell 153, 590-600. PMID: 23622243 10. Carvalho, S. et al. (2014) eLife 3, e02482. PMID: 24843002 |
| 注意事項 | For research use only |
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