Anti-Human SETD2 IP Recombinant Antibody (SAA0221) (HP214013)

Name: Anti-Human SETD2 IP Recombinant Antibody (SAA0221)
Brand: abinScience
SKU: HP214013
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HP214013

Species reactivity

Human

Applications

ELISA, IF, IP, IP-MS

Host species

Mouse

Isotype

IgG

Clone ID

SAA0221

Clonality

Monoclonal

Target

Protein-lysine N-methyltransferase SETD2, HYPB, Histone-lysine N-methyltransferase SETD2, KMT3A, SET2, KIAA1732, Huntingtin yeast partner B, HIF-1, Huntingtin-interacting protein B, Huntingtin-interacting protein 1, HIP-1, Lysine N-methyltransferase 3A, SET domain-containing protein 2, p231HBP, SETD2, HIF1, hSET2

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q9BYW2

RRID

Anti-Human SETD2 IP Antibody (SAA0221) (abinScience Cat# HP214013, RRID:AB_3729755)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Histone-lysine N-methyltransferase SETD2 is a ~287 kDa protein. Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate. It is capable of trimethylating unmethylated H3K36 (H3K36me0) in vitro. Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation. Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A. Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction.

1. Sun, XJ. et al. (2005) The Journal of biological chemistry 280, 35261-71. PMID: 16118227

2. Yoh, SM. et al. (2008) Genes & development 22, 3422-34. PMID: 19141475

3. Hahn, MA. et al. (2011) PloS one 6, e18844. PMID: 21526191

4. de Almeida, SF. et al. (2011) Nature structural & molecular biology 18, 977-83. PMID: 21792193

5. Zheng, W. et al. (2012) Journal of the American Chemical Society 134, 18004-14. PMID: 23043551

6. Yang, S. et al. (2016) Genes & development 30, 1611-6. PMID: 27474439

7. Yuan, W. et al. (2009) The Journal of biological chemistry 284, 15701-7. PMID: 19332550

8. Carvalho, S. et al. (2013) Nucleic acids research 41, 2881-93. PMID: 23325844

9. Li, F. et al. (2013) Cell 153, 590-600. PMID: 23622243

10. Carvalho, S. et al. (2014) eLife 3, e02482. PMID: 24843002

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human SETD2 IP Antibody (SAA0221)

References

Datasheet

Anti-Human SETD2 IP Recombinant Antibody (SAA0221).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-Human SETD2 IP Recombinant Antibody (SAA0221) (HP214013)

Overview

Images

References

Datasheet

SDS

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