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| カタログ番号 | HP532012 |
|---|---|
| 説明 |
Recombinant Human SIRT5 Protein, N-His (HP532012) expressed in E. coli, spanning Met40-Ser310. Purity: >90% by SDS-PAGE.
Highlights
|
| 発現システム | E. coli |
| アクセッション番号 | Q9NXA8 |
| タンパク質長 | Met40-Ser310 |
| アプリケーション | ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress |
| 種 | Homo sapiens (Human) |
| 性質 | Recombinant |
| エンドトキシンレベル | Please contact with the lab for this information. |
| 純度 | >90% as determined by SDS-PAGE. |
| 予測分子量 | 31.72 kDa |
| 形態 | Lyophilized |
| 保存バッファー | Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. データシートのハードコピーまたはロット固有のCOAに記載された具体的なバッファー情報を参照してください。 |
| 再構成 | Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended. |
| 発送 | In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise. |
| 安定性と保存 | Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt. |
| 別名 | SIR2-like protein 5, SIRT5, SIR2L5, Regulatory protein SIR2 homolog 5, NAD-dependent protein deacylase sirtuin-5, mitochondrial |
| 背景 | NAD-dependent protein deacylase sirtuin-5, mitochondrial (SIRT5) is a ~33 kDa protein. NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Activates SHMT2 by mediating its desuccinylation. Modulates ketogenesis through the desuccinylation and activation of HMGCS2. 1. Peng, C. et al. (2011) Molecular & cellular proteomics : MCP 10, M111.012658. PMID: 21908771 2. Du, J. et al. (2011) Science (New York, N.Y.) 334, 806-9. PMID: 22076378 3. Tan, M. et al. (2014) Cell metabolism 19, 605-17. PMID: 24703693 4. Yang, X. et al. (2018) Cancer research 78, 372-386. PMID: 29180469 5. Lin, ZF. et al. (2013) Biochemical and biophysical research communications 441, 191-5. PMID: 24140062 |
| 注意事項 | For research use only. |
SDS-PAGE for Recombinant Human SIRT5 Protein, N-His
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中国武漢市深敦寺路666号C棟、武漢、430206
