Anti-Human SIRT5 Polyclonal Antibody (HP532014)

Name: Anti-Human SIRT5 Polyclonal Antibody
Brand: abinScience
SKU: HP532014
Price: 168 USD
Availability: InStock

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Overview
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References
Datasheet
SDS

Overview

Catalog No.

HP532014

Description

Anti-Human SIRT5 Polyclonal Antibody (HP532014) is a rabbit polyclonal antibody detecting SIRT5 in ELISA, IHC, WB. Suitable for Human, Mouse, Dog, Rat, and Bovine.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.
●Multi-Species — Cross-reactive for translational research.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SIRT5 (Met40-Ser310).

Target

SIR2-like protein 5, SIRT5, SIR2L5, Regulatory protein SIR2 homolog 5, NAD-dependent protein deacylase sirtuin-5, mitochondrial

Purification

Purified by antigen affinity column.

Accession

Q9NXA8

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

NAD-dependent protein deacylase sirtuin-5, mitochondrial (SIRT5) is a ~33 kDa protein. NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Activates SHMT2 by mediating its desuccinylation. Modulates ketogenesis through the desuccinylation and activation of HMGCS2.

1. Peng, C. et al. (2011) Molecular & cellular proteomics : MCP 10, M111.012658. PMID: 21908771

2. Du, J. et al. (2011) Science (New York, N.Y.) 334, 806-9. PMID: 22076378

3. Tan, M. et al. (2014) Cell metabolism 19, 605-17. PMID: 24703693

4. Yang, X. et al. (2018) Cancer research 78, 372-386. PMID: 29180469

5. Lin, ZF. et al. (2013) Biochemical and biophysical research communications 441, 191-5. PMID: 24140062

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-Human SIRT5 polyclonal antibody at 1ug/mL on recombinant Human SIRT5 (Catalog No. HP532012).

References

Datasheet