ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies
Homo sapiens (Human)
Naturaleza
Recombinant
Nivel de endotoxinas
Please contact with the lab for this information.
Pureza
>90% as determined by SDS-PAGE.
Peso molecular predicho
37.65 kDa
Forma
Lyophilized
Buffer de almacenamiento
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol.
Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución
Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet.
RECONSTITUTION PROTOCOL
1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature.
2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial.
3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service.
4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down.
5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using.
6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos
Cyclin-A/CDK2-associated protein p45, F-box/LRR-repeat protein 1, S-phase kinase-associated protein 2, SKP2, p45skp2, FBXL1, F-box protein Skp2
Fondo
S-phase kinase-associated protein 2 (SKP2) is a ~47 kDa protein. Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, NBN, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1.
1. Yu, ZK. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 11324-9. PMID: 9736735
2. Méndez, J. et al. (2002) Molecular cell 9, 481-91. PMID: 11931757
3. Tedesco, D. et al. (2002) Genes & development 16, 2946-57. PMID: 12435635
4. von der Lehr, N. et al. (2003) Molecular cell 11, 1189-200. PMID: 12769844
5. Li, X. et al. (2003) The Journal of biological chemistry 278, 30854-8. PMID: 12840033
6. Tokarz, S. et al. (2004) The Journal of biological chemistry 279, 46424-30. PMID: 15342634
7. Huang, H. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 1649-54. PMID: 15668399
8. Jiang, H. et al. (2005) Molecular cell 18, 699-709. PMID: 15949444
9. Barboric, M. et al. (2005) Journal of virology 79, 11135-41. PMID: 16103164
10. Wang, W. et al. (2005) Biochemistry 44, 14553-64. PMID: 16262255
Nota
For research use only.
Imágenes
SDS-PAGE
SDS-PAGE for Recombinant Human SKP2 Protein, N-His
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