Anti-SKP2 Polyclonal Antibody (HC635014)

Name: Anti-SKP2 Polyclonal Antibody
Brand: abinScience
SKU: HC635014
Price: 168 USD
Availability: InStock

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SDS

Overview

Catalog No.

HC635014

Description

Anti-SKP2 Polyclonal Antibody (HC635014) is a rabbit polyclonal antibody detecting SKP2 in ELISA, IHC, WB. Suitable for Human.

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Human

Applications

ELISA, IHC, WB

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Immunogen

E. coli - derived recombinant Human SKP2 (Lys43-Leu354).

Target

Cyclin-A/CDK2-associated protein p45, F-box/LRR-repeat protein 1, S-phase kinase-associated protein 2, SKP2, p45skp2, FBXL1, F-box protein Skp2

Purification

Purified by antigen affinity column.

Accession

Q13309

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

S-phase kinase-associated protein 2 (SKP2) is a ~47 kDa protein. Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription. Specifically recognizes phosphorylated CDKN1B/p27kip and is involved in regulation of G1/S transition. Degradation of CDKN1B/p27kip also requires CKS1. Recognizes target proteins ORC1, CDT1, RBL2, KMT2A/MLL1, CDK9, RAG2, NBN, FOXO1, UBP43, YTHDF2, and probably MYC, TOB1 and TAL1. Degradation of TAL1 also requires STUB1.

1. Yu, ZK. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 11324-9. PMID: 9736735

2. Méndez, J. et al. (2002) Molecular cell 9, 481-91. PMID: 11931757

3. Tedesco, D. et al. (2002) Genes & development 16, 2946-57. PMID: 12435635

4. von der Lehr, N. et al. (2003) Molecular cell 11, 1189-200. PMID: 12769844

5. Li, X. et al. (2003) The Journal of biological chemistry 278, 30854-8. PMID: 12840033

6. Tokarz, S. et al. (2004) The Journal of biological chemistry 279, 46424-30. PMID: 15342634

7. Huang, H. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 1649-54. PMID: 15668399

8. Jiang, H. et al. (2005) Molecular cell 18, 699-709. PMID: 15949444

9. Barboric, M. et al. (2005) Journal of virology 79, 11135-41. PMID: 16103164

10. Wang, W. et al. (2005) Biochemistry 44, 14553-64. PMID: 16262255

Note

For research use only.

Images

References

Datasheet

Anti-SKP2 Polyclonal Antibody.pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

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Anti-SKP2 Polyclonal Antibody (HC635014)

Overview

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References

Datasheet

SDS

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