Human SLC12A2 Recombinant Protein (N-His) (HX014012)

Name: Human SLC12A2 Recombinant Protein (N-His)
Brand: abinScience
SKU: HX014012
Price: 211 USD
Availability: InStock

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1mg

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SDS

Vista general

Número de catálogo	HX014012
Descripción	Recombinant Human SLC12A2 Protein, N-His (HX014012) expressed in E. coli, spanning Asn1002-Ser1212. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Sistema de expresión	E. coli
Número de acceso	P55011
Longitud de proteína	Asn1002-Ser1212
Aplicaciones	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Especies	Homo sapiens (Human)
Naturaleza	Recombinant
Nivel de endotoxinas	Please contact with the lab for this information.
Pureza	>90% as determined by SDS-PAGE.
Peso molecular predicho	26.82 kDa
Forma	Lyophilized
Buffer de almacenamiento	Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Consulte la información específica del buffer en la copia impresa del datasheet o en el COA específico del lote.
Reconstitución	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Envío	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Estabilidad y almacenamiento	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Nombres alternativos	Solute carrier family 12 member 2, Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 2, SLC12A2, NKCC1
Fondo	Solute carrier family 12 member 2 (SLC12A2/NKCC1) is a ~131 kDa protein. Cation-chloride cotransporter which mediates the electroneutral transport of chloride, potassium and/or sodium ions across the membrane. Plays a vital role in the regulation of ionic balance and cell volume. 1. Vitari, AC. et al. (2006) The Biochemical journal 397, 223-31. PMID: 16669787 2. Yang, X. et al. (2020) Nature communications 11, 1016. PMID: 32081947 3. Mutai, H. et al. (2020) PLoS genetics 16, e1008643. PMID: 32294086 4. Zhang, S. et al. (2021) Communications biology 4, 226. PMID: 33597714 5. Zhao, Y. et al. (2022) Nature communications 13, 2747. PMID: 35585053 6. Neumann, C. et al. (2022) The EMBO journal 41, e110169. PMID: 36239040 7. Moseng, MA. et al. (2022) Science advances 8, eabq0952. PMID: 36306358 8. Payne, JA. et al. (1995) The Journal of biological chemistry 270, 17977-85. PMID: 7629105
Nota	For research use only.

Imágenes

SDS-PAGE

SDS-PAGE for Recombinant Human SLC12A2 Protein, N-His

Referencias

Datasheet

Human SLC12A2 Recombinant Protein (N-His).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

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